The Molecular Biology Laboratory at TSBS focuses on understanding the fundamental DNA → RNA → Protein pathway through qualitative and quantitative assays.
Equipment & instruments
- Thermocycler (PCR device)
- UV-Visible spectrophotometer
- Digital precision balance and pH meter
- Horizontal and vertical slab gels with electrophoresis power packs
- UV trans-illuminator
- High-speed and refrigerated centrifuges
- Variable-volume auto pipettes
- ELISA reader
- Orbital shaker, homogenizer and gel rocker
Core curriculum experiments
Standard experiments include SDS-PAGE, genomic DNA extraction, agarose gel electrophoresis, blotting techniques (Southern, Northern, Western), RNA/mRNA isolation, ELISA, plasmid work, bacterial transformation, and PCR/RFLP/RAPD analysis.
Beyond-syllabus work
Students may pursue amylase analysis via SDS-PAGE and Western transfer, enzyme kinetics using ELISA readers, organellar DNA isolation from plants, and native-PAGE conformational protein studies.
